DETERMINATION OF GLUCOSE-OXIDASE BY MEANS OF THE CHEMILUMINESCENCE OF CYPRIDINA LUCIFERIN ANALOG

被引：5
|
作者：
MITANI, M [1 ]
KOINUMA, Y [1 ]
KOSUGI, M [1 ]
机构：
[1] GUNMA UNIV,FAC ENGN,DEPT CHEM,KIRYU,GUNMA 376,JAPAN
来源：
ANALYTICAL SCIENCES | 1994年 / 10卷 / 02期
关键词：
LUCIFERIN ANALOG; GLUCOSE OXIDASE; CHEMILUMINESCENCE;
D O I：
10.2116/analsci.10.267
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
FeCl2 was found to be a useful catalyst for the determination of H2O2 based on the chemiluminescence of a Cypridina luciferin analogue, 2-methyl-6-phenyl-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (MCLA). By this method, H2O2 was detected in a range from 300 pmol/ml to 10 nmol/ml. In addition, glucose oxidase (GOD) was determined in a range from 1.18 x 10(-5) units/ml (280 amol/ml) to 7.56 x 10(-4) units/ml (17.9 fmol/ml) when GOD was incubated with glucose for 10 min at 34-degrees-C.
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页码：267 / 269
页数：3
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