DETERMINATION OF GLUCOSE-OXIDASE BY MEANS OF THE CHEMILUMINESCENCE OF CYPRIDINA LUCIFERIN ANALOG

被引：5

作者：

MITANI, M ^{[1
]}

KOINUMA, Y ^{[1
]}

KOSUGI, M ^{[1
]}

机构：

[1] GUNMA UNIV,FAC ENGN,DEPT CHEM,KIRYU,GUNMA 376,JAPAN

来源：

ANALYTICAL SCIENCES | 1994年 / 10卷 / 02期

关键词：

LUCIFERIN ANALOG; GLUCOSE OXIDASE; CHEMILUMINESCENCE;

D O I：

10.2116/analsci.10.267

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

FeCl2 was found to be a useful catalyst for the determination of H2O2 based on the chemiluminescence of a Cypridina luciferin analogue, 2-methyl-6-phenyl-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (MCLA). By this method, H2O2 was detected in a range from 300 pmol/ml to 10 nmol/ml. In addition, glucose oxidase (GOD) was determined in a range from 1.18 x 10(-5) units/ml (280 amol/ml) to 7.56 x 10(-4) units/ml (17.9 fmol/ml) when GOD was incubated with glucose for 10 min at 34-degrees-C.

引用

页码：267 / 269

页数：3

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