CLONING AND SEQUENCING SHOW THAT 4-HYDROXYBENZOATE HYDROXYLASE (POBA) IS REQUIRED FOR UPTAKE OF 4-HYDROXYBENZOATE IN RHIZOBIUM-LEGUMINOSARUM

被引：23
作者：
WONG, CM [1 ]
DILWORTH, MJ [1 ]
GLENN, AR [1 ]
机构：
[1] MURDOCH UNIV, SCH BIOL & ENVIRONM SCI, NITROGEN FIXAT RES GRP, MURDOCH, WA 6150, AUSTRALIA
来源：
MICROBIOLOGY-SGM | 1994年 / 140卷
关键词：
RHIZOBIUM LEGUMINOSARUM; AROMATIC SUBSTRATE UPTAKE; 4-HYDROXYBENZOATE; SEQUENCE OF POBA;
D O I：
10.1099/00221287-140-10-2775
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Mutants of Rhizobium leguminosarum bv. viciae MNF300 and R. leguminosarum bv. trifolii WU95 unable to accumulate 4-hydroxybenzoate lack 4-hydroxybenzoate hydroxylase. The capacity of these mutants to take up and grow on 4-hydroxybenzoate was restored by a 2.0 kb EcoRI-PstI DNA fragment. This contained only one ORF which had over 60% DNA sequence similarity with the structural gene for 4-hydroxybenzoate hydroxylase (pobA) from Pseudomonas spp. and Acinetobacter. Reported effects of metabolic inhibitors and substrate analogues on the apparent uptake of 4-hydroxybenzoate have now been shown to be due to their direct effect on 4-hydroxybenzoate hydroxylase. We propose that uptake of 4-hydroxybenzoate is via a metabolic 'drag' mechanism dependent on the activity of the pobA gene product.
引用
收藏
页码：2775 / 2786
页数：12
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[31]   Esterification of sodium 4-hydroxybenzoate by ultrasound-assisted solid-liquid phase-transfer catalysis using dual-site phase-transfer catalyst [J].
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Chu, Wei-Ming .
ULTRASONICS SONOCHEMISTRY, 2014, 21 (01) :395-400
[32]   Comparative genome analysis among Variovorax species and genome guided aromatic compound degradation analysis emphasizing 4-hydroxybenzoate degradation in Variovorax sp. PAMC26660 [J].
Nisha Ghimire ;
Byeollee Kim ;
Chang-Muk Lee ;
Tae-Jin Oh .
BMC Genomics, 23
[33]   Crystal structure of l-4-oxidobenzoato-kappa O-2(1) : O-4 bis[bis(1,10-phenanthroline-kappa(2) N, N ') copper(II)] bis(4-hydroxybenzoate) 7.5-hydrate [J].
Su, Jian-Rong ;
Li, Yu .
ACTA CRYSTALLOGRAPHICA SECTION E-CRYSTALLOGRAPHIC COMMUNICATIONS, 2016, 72 :1396-+
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