CONSTRUCTION OF A BINDING-SITE FOR HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GP120 IN RAT CD4

被引：26

作者：

SCHOCKMEL, GA

SOMOZA, C

DAVIS, SJ

WILLIAMS, AF

HEALEY, D

机构：

[1] UNIV OXFORD, SIR WILLIAM DUNN SCH PATHOL, MRC, CELLULAR IMMUNOL UNIT, OXFORD OX1 3RE, ENGLAND

[2] UNIV CAMBRIDGE, DEPT PATHOL, CAMBRIDGE CB2 1QP, ENGLAND

来源：

JOURNAL OF EXPERIMENTAL MEDICINE | 1992年 / 175卷 / 01期

关键词：

D O I：

10.1084/jem.175.1.301

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The human immunodeficiency virus (HIV-1) infects T lymphocytes via an interaction between the virus envelope glycoprotein gp120 and the CD4 antigen of T helper cells. Previous studies demonstrated that mutations in various regions of CD4 domain 1 lead to the loss of gp120 binding. In the present study the gp120 binding site was constructed in rat CD4 by replacing rat with human CD4 sequence. A series of mutants was constructed the best of which bound gp120 with an affinity only twofold less than that of human CD4. The data indicate that the gp120 binding site of human CD4 is constituted by residues 33-58 of domain 1.

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页码：301 / 304

页数：4

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