GENETIC APPROACH TO THE STUDY OF EPIDEMIOLOGY AND PATHOGENESIS OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS IN LOCALIZED JUVENILE PERIODONTITIS

被引:46
作者
DIRIENZO, JM [1 ]
SLOTS, J [1 ]
机构
[1] UNIV PENN,SCH DENT MED,DEPT PERIODONT,PHILADELPHIA,PA 19104
关键词
JUVENILE PERIODONTITIS; EPIDEMIOLOGY; VIRULENCE; RESTRICTION FRAGMENT-LENGTH POLYMORPHISM; DNA PROBES; ACTINOBACCILLUS-ACTINOMYCETEMCOMITANS;
D O I
10.1016/0003-9969(90)90134-V
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Actinobacillus actinomycetemcomitans isolates from periodontal pockets were examined for restriction fragment-length polymorphism using a characterized 4.7-kb DNA probe. A total of 6 patterns of RFLP was found in 133 isolates originating from 12 subjects. No relatedness was found between RFLP types and serotypes. Different periodontal sites within the same subject and different individuals within the same family sometimes showed only one type of A. actinomycetemcomitans RFLP. When members among the same family showed 2 RFLP types, children were always infected with the A. actinomycetemcomitans strains found in at least one of the parents. These findings support the concept of familial spread of A. actinomycetemcomitans. A. actinomycetemcomitans RFLP type B, corresponding to reference strain JP2, seems to be particularly virulent, as indicated from the presence of RFLP type B in 3 subjects who converted from a healthy periodontal state to localized juvenile periodontitis. RFLP type B was not detected in any of the 21 A. actinomycetemcomitans-infected patients with adult periodontitis. The RFLP method seems to be useful in determining the epidemiology and possibly the potential virulence of periodontal strains of A. actinomycetemcomitans.
引用
收藏
页码:S79 / S84
页数:6
相关论文
共 18 条
[1]  
Aicoforado G A, 1987, Oral Microbiol Immunol, V2, P35, DOI 10.1111/j.1399-302X.1987.tb00267.x
[2]   CASE FOR PERIODONTOSIS AS A CLINICAL ENTITY [J].
BAER, PN .
JOURNAL OF PERIODONTOLOGY, 1971, 42 (08) :516-&
[3]   PROBE-SPECIFIC DNA FINGERPRINTING APPLIED TO THE EPIDEMIOLOGY OF LOCALIZED JUVENILE PERIODONTITIS [J].
DIRIENZO, JM ;
CORNELL, S ;
KAZOROSKI, L ;
SLOTS, J .
ORAL MICROBIOLOGY AND IMMUNOLOGY, 1990, 5 (02) :49-56
[4]   HOST RESPONSES IN PERIODONTAL-DISEASES [J].
GENCO, RJ ;
SLOTS, J .
JOURNAL OF DENTAL RESEARCH, 1984, 63 (03) :441-451
[5]  
LINDHE J, 1989, TXB CLIN PERIODONTOL, P193
[6]  
Moller A.J.R., 1966, ODONTOL TIDSKR, V74, P1
[7]   POSSIBLE TRANSMITTANCE OF A-ACTINOMYCETEMCOMITANS FROM A DOG TO A CHILD WITH RAPIDLY DESTRUCTIVE PERIODONTITIS [J].
PREUS, HR ;
OLSEN, I .
JOURNAL OF PERIODONTAL RESEARCH, 1988, 23 (01) :68-71
[8]   THE PRESENCE OF PHAGE-INFECTED ACTINOBACILLUS-ACTINOMYCETEMCOMITANS IN LOCALIZED JUVENILE PERIODONTITIS PATIENTS [J].
PREUS, HR ;
OLSEN, I ;
NAMORK, E .
JOURNAL OF CLINICAL PERIODONTOLOGY, 1987, 14 (10) :605-609
[9]   BLACK-PIGMENTED BACTEROIDES SPECIES, CAPNOCYTOPHAGA SPECIES, AND ACTINOBACILLUS-ACTINOMYCETEMCOMITANS IN HUMAN PERIODONTAL-DISEASE - VIRULENCE FACTORS IN COLONIZATION, SURVIVAL, AND TISSUE DESTRUCTION [J].
SLOTS, J ;
GENCO, RJ .
JOURNAL OF DENTAL RESEARCH, 1984, 63 (03) :412-421
[10]   SELECTIVE MEDIUM FOR ISOLATION OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS [J].
SLOTS, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 1982, 15 (04) :606-609