LIQUID-CHROMATOGRAPHIC SEPARATION OF THE ANOMERS AND ISOMERS OF 2'-DEOXYURIDINE

被引：4

作者：

VANSCHEPDAEL, A

VANAERSCHOT, A

HERDEWIJN, P

ROETS, E

HOOGMARTENS, J

机构：

[1] Laboratorium voor Farmaceutische Chemie, Instituut voor Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Leuven, B-3000

来源：

CHROMATOGRAPHIA | 1992年 / 33卷 / 11-12期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; 2'-DEOXYURIDINE; ANOMER SEPARATION; ISOMER SEPARATION;

D O I：

10.1007/BF02262250

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A liquid chromatographic assay method was developed to separate 2-deoxyuridine from its anomer and pentopyranosyl isomers. The influence of the pH of the mobile phase, the type of organic modifier and the column temperature on the chromatographic parameters was systematically investigated. Using a Hypersil C18 (5-mu-m) column at 10-degrees-C, the following mobile phase was found suitable: tetrahydrofuran - 0.2 M potassium phosphate buffer pH 4.0 - water (0.1 : 5 : 94.9, v/v).

引用

页码：571 / 574

页数：4

共 11 条

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