DETECTION OF APOLIPROPROTEIN-E POLYMORPHISMS USING PCR ASO PROBES AND SOUTHERN TRANSFER - APPLICATION FOR ROUTINE USE

被引：8

作者：

HOULSTON, RS

WENHAM, PR

HUMPHRIES, SE

机构：

[1] WESTERN GEN HOSP,DEPT CLIN CHEM,CREWE RD,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND

[2] CHARING CROSS SUNLEY MED RES CTR,ARTERIAL DIS RES GRP,LONDON,ENGLAND

来源：

CLINICA CHIMICA ACTA | 1990年 / 189卷 / 02期

关键词：

Allele specific oligonucleotide; Polymerase chain reaction; Southern blotting;

D O I：

10.1016/0009-8981(90)90086-8

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The detection of apolipoprotein E genotypes is of importance both for diagnostic and research purposes. We have previously used the polymerase chain reaction to amplify a specific region of the apolipoprotein E gene which, when used in conjunction with allele specific oligonuleotide probes, permits the detection of the six common apolipoprotein genotypes. In our present report we have modified the above procedure by immobilising the amplified DNA using Southern blotting. This enables the technique to be used in routine laboratories with minimal expenditure and little risk of cross-contamination between samples. Futhermore, it is inherently robust and may be rapidly performed. © 1990.

引用

页码：153 / 158

页数：6

共 13 条

[1] APOLIPOPROTEIN-(APO)-E GENOTYPES BY POLYMERASE CHAIN-REACTION AND ALLELE-SPECIFIC OLIGONUCLEOTIDE PROBES - NO DETECTABLE LINKAGE DISEQUILIBRIUM BETWEEN APO-E AND APO-CII [J].