REGULATION OF ISOLEUCINE-VALINE BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE .2. IDENTIFICATION AND CHARACTERIZATION OF MUTANTS LACKING ACETOHYDROXYACID SYNTHETASE

被引：60

作者：

MAGEE, PT

DEROBICH.H

机构：

[1] Laboratoire d'Enzymologie du C.N.R.S., Gif-Sur-Yvette

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1968年 / 3卷 / 04期

关键词：

D O I：

10.1111/j.1432-1033.1967.tb19559.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acetohydroxyacid synthetase activity has been characterized, independently of the acetoin‐forming system in Saccharomyces cerevisiae by a differential colorimetric assay. Maximum pH and Km values have been established for both enzymes with pyruvate as the substrate. Acetolactate formation by acetohydroxyacid synthetase has been shown directly by the separation of dinitrophenylosazones by thin‐layer chromatography. Evidence for the biosynthetic role of the activity measured resides in its presence in wild‐type strains, in its absence in the is2 class of mutants (isoleucine‐valine auxotrophs) and in its reappearance in prototrophic revertants of these mutants. Copyright © 1968, Wiley Blackwell. All rights reserved

引用

页码：502 / &

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[2]

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[3]

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[5]