METABOLISM OF 2,4,5,2',4',5'-HEXACHLOROBIPHENYL WITH LIVER-MICROSOMES OF PHENOBARBITAL-TREATED DOG - THE POSSIBLE FORMATION OF PCB 2,3-ARENE OXIDE INTERMEDIATE

被引:43
作者
ARIYOSHI, N
KOGA, N
OGURI, K
YOSHIMURA, H
机构
[1] KYUSHU UNIV,FAC PHARMACEUT SCI,3-1-1 MAIDASHI,HIGASHI KU,FUKUOKA 812,JAPAN
[2] NAKAMURA GAKUEN COLL,DEPT FOOD & NUTR,JOHNAN KU,FUKUOKA 81401,JAPAN
关键词
D O I
10.3109/00498259209053156
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
1. Metabolism of 2,4,5,2',4',5'-hexachlorobiphenyl (HCB) was investigated in vitro using liver microsomes of one male beagle dog after phenobarbital treatment. 2. Three major metabolites were isolated and identified as 3-hydroxy-2,4,5,2',4',5'-HCB, 2-hydroxy-4,5,2',4',5'-pentachlorobiphenyl (PenCB), and 2-hydroxy-3,4,5,2',4',5'-HCB, by comparison of g.l.c.-mass spectrometry and H-1-n.m.r. data with those of authentic samples. 3. 2-Hydroxy-3,4,5,2',4',5'-HCB was found as a metabolite of 2,4,5,2',4',5'-HCB for the first time using dog liver microsomes. Present results indicate that this metabolite and the dechlorinated PenCB are derived from a metabolic intermediate, namely, 2,3-epoxy-2,4,5,2',4',5'-HCB. 2,3-Epoxide formation is a new metabolic pathway of PCB.
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页码:1275 / 1290
页数:16
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