MOLECULAR-CLONING OF CDNA-ENCODING HUMAN LANOSTEROL SYNTHASE

被引：0

作者：

SUNG, CK ^{[1
]}

SHIBUYA, M ^{[1
]}

SANKAWA, U ^{[1
]}

EBIZUKA, Y ^{[1
]}

机构：

[1] UNIV TOKYO, FAC PHARMACEUT SCI, BUNKYO KU, TOKYO 113, JAPAN

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 1995年 / 18卷 / 10期

关键词：

LANOSTEROL SYNTHASE; CDNA; HUMAN LIVER; PCR; 2,3-OXIDOSQUALENE; LANOSTEROL; CYCLASE;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A cDNA encoding human lanosterol synthase, the enzyme responsible for the backbone formation step in sterol biosynthesis, was cloned by extensive application of PCRs. Five degenerate oligonucleotide primers (139S, 440S, 528A, 575A and 712A) corresponding to the homologous amino acid sequences among the known 2,3-oxidosqualene cyclase(OSC) were designed. PCR with one pair (440S and 528A) of five primers yielded a 285-bp fragment. PCRs with the primers based on the obtained fragment and the degenerate primers (139S and 712A) gave longer fragments. Finally, full nucleotide sequence of cDNA was obtained by a ''rapid amplification of cDNA ends'' (RACE) method.

引用

页码：1459 / 1461

页数：3

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