IMMUNOLOGICAL DETECTION OF PROHORMONE CONVERTASES IN 2 DIFFERENT PROGLUCAGON PROCESSING CELL-LINES

被引：19

作者：

BLACHE, P ^{[1
]}

LENGUYEN, D ^{[1
]}

BOEGNERLEMOINE, C ^{[1
]}

COHENSOLAL, A ^{[1
]}

BATAILLE, D ^{[1
]}

KERVRAN, A ^{[1
]}

机构：

[1] CCIPE,INSERM,UNITE 376,F-34094 MONTPELLIER,FRANCE

来源：

FEBS LETTERS | 1994年 / 344卷 / 01期

关键词：

GLUCAGON; OXYNTOMODULIN; ALPHA-TC1-6; CELL; STC-1; PROHORMONE CONVERTASE; IMMUNOBLOT; RADIOIMMUNOASSAY;

D O I：

10.1016/0014-5793(94)00371-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The distribution of the prohormone convertases, PC1/3, PC2 and PC5/6, was determined by immunoblotting in two cell lines. In alpha TC1-6 cells, the proglucagon processing occured according to the pancreatic A-cell type. In STC-1 cells, proglucagon was processed in a manner reminiscent of the intestinal L-cell type. PC1/3 was undetectable in both proglucagon processing cell lines whereas PC2 displayed a strong immunostaining in the alpha TC1-6 cells and was barely detectable in the STC-1 cells. PC5/6 was detected as a 70 kDa protein in both cell lines. These results suggest a possible role of PC2 in the processing of proglucagon into glucagon in the A-cells, whereas in L-cells it would require still undetermined endoproteases.

引用

页码：65 / 68

页数：4

共 31 条

[1] PC1 AND PC2 ARE PROPROTEIN CONVERTASES CAPABLE OF CLEAVING PROOPIOMELANOCORTIN AT DISTINCT PAIRS OF BASIC RESIDUES [J].