IGG AUTOANTIBODIES TO C1Q DO NOT DETECTABLY INFLUENCE COMPLEMENT ACTIVATION INVIVO AND INVITRO IN SYSTEMIC LUPUS-ERYTHEMATOSUS

被引:23
作者
SIEGERT, CEH [1 ]
DAHA, MR [1 ]
LOBATTO, S [1 ]
VANDERVOORT, EAM [1 ]
BREEDVELD, FC [1 ]
机构
[1] LEIDEN UNIV HOSP,DEPT NEPHROL,2333 AA LEIDEN,NETHERLANDS
关键词
C1Q ANTIBODY; COMPLEMENT; IMMUNE COMPLEXES; SYSTEMIC LUPUS ERYTHEMATOSUS;
D O I
10.1007/BF02918613
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The influence of IgG antibodies to C1q (C1qAb) on activation of the classical pathway of the complement system was investigated in patients with systemic lupus erythematosus (SLE). In in vivo experiments, a prototype for immune complexes was administered intravenously to 14 patients and 9 healthy controls. Eight SLE patients had increased C1qAb titers. The increase of C3a levels, which was measured as a parameter of C1 activation, was significantly lower in SLE patients than in the healthy controls (p = 0.01). No correlation was found between C3a increases and C1qAb titers. In in vitro experiments the influence on C1 activation of monomeric IgG isolated from serum of 11 SLE patients, 7 of whom had increased C1qAb titers, was measured in a C4 consumption assay. The presence of C1qAb did not influence C4 consumption. The results demonstrate that C1qAb do not influence C1 activation by immune complexes in SLE patients.
引用
收藏
页码:91 / 97
页数:7
相关论文
共 30 条
[1]  
AGNELLO V, 1971, J EXP MED, V134, pS228
[2]   EVIDENCE FOR THE PRESENCE OF AUTOANTIBODIES TO THE COLLAGEN-LIKE PORTION OF C1Q IN SYSTEMIC LUPUS-ERYTHEMATOSUS [J].
ANTES, U ;
HEINZ, HP ;
LOOS, M .
ARTHRITIS AND RHEUMATISM, 1988, 31 (04) :457-464
[3]  
COOPER KM, 1983, CLIN EXP IMMUNOL, V52, P407
[4]  
DAHA MR, 1982, CLIN EXP IMMUNOL, V48, P733
[5]  
DOEKES G, 1982, IMMUNOLOGY, V45, P705
[6]  
GAITHER TA, 1974, J IMMUNOL, V113, P574
[7]  
GOLAN MD, 1982, J IMMUNOL, V129, P445
[8]  
GREISMAN SG, 1987, J IMMUNOL, V138, P739
[9]   MONOCLONAL-ANTIBODIES AGAINST COMPONENTS OF THE CLASSICAL PATHWAY OF COMPLEMENT [J].
HEINZ, HP ;
LOOS, M .
COMPLEMENT AND INFLAMMATION, 1989, 6 (03) :166-174
[10]  
Heinz HP, 1989, BEHRING I MITT, V84, P20