DETERMINATION OF THE DNA CONFORMATION OF THE SIMIAN VIRUS-40 (SV40) ENHANCER IN SV40 MINICHROMOSOMES

被引：2

作者：

MARTINEZBALBAS, A ^{[1
]}

CASASNOVAS, JM ^{[1
]}

AZORIN, F ^{[1
]}

机构：

[1] ESCUELA TECN SUPER INGN IND,CSIC,CTR INVEST & DESARROLLO,QUIM MACROMOLEC GRP,BARCELONA,SPAIN

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 188卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1990.tb15399.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The simian virus 40 (SV40) enhancer contains three 8‐bp purine‐pyrimidine alternating sequences which are known to adopt the left‐handed Z‐DNA conformation in vitro. In this paper, we have undertaken the determination of the DNA conformation adopted by these Z‐motifs in the SV40 minichromosome. We have analyzed the presence of Z‐DNA through the change in linkage which should accompany formation of this left‐handed conformation. Our results indicate that, regardless of the precise moment of the viral lytic cycle at which minichromosomes are harvested and the condition of the transfected DNA, either relaxed or negatively supercoiled, none of the three Z motifs of the SV40 enhancer exist to a significant extent as Z‐DNA in SV40 minichromosomes. The SV40 enhancer adopts predominantly a right‐handed B‐DNA conformation in vivo. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：269 / 273

页数：5

共 27 条

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