SEQUENCING OF ANTISENSE DNA ANALOGS BY CAPILLARY GEL-ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION

被引：21

作者：

BELENKY, A ^{[1
]}

SMISEK, DL ^{[1
]}

COHEN, AS ^{[1
]}

机构：

[1] HYBRIDON INC,WORCESTER,MA 01605

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 700卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)01092-S

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method using capillary gel electrophoresis with laser-induced fluorescence detection is described which permits complete sequence determination of antisense DNA analogues of unknown sequence. This method, originally created as a tool to confirm the sequence of antisense oligonucleotides being developed as therapeutic drugs, utilizes data collected under a range of experimental conditions described by the Ogston model as applied to gel electrophoresis. A linear relationship independent of experimental conditions between the relative electrophoretic migration time and the oligonucleotide base number was observed and is shown to be consistent with a simplified version of this model and can be used to facilitate the sequence determination.

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页码：137 / 149

页数：13

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