ISOLATION OF FUNCTIONAL POLYMORPHONUCLEAR LEUKOCYTES FROM RHESUS-MONKEYS USING DISCONTINUOUS FICOLL-HYPAQUE DENSITY GRADIENTS

被引：0

作者：

TANIUCHI, S ^{[1
]}

机构：

[1] UNIV CALIF DAVIS,SCH MED,DEPT PATHOL,DAVIS,CA 95616

来源：

JOURNAL OF MEDICAL PRIMATOLOGY | 1991年 / 20卷 / 05期

关键词：

CHEMOTAXIS; PMN; PURIFICATION; SEPARATION; MATURE PMN; IMMATURE PMN;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A simple, rapid method for separating polymorphonuclear leukocytes (PMNs) from adult rhesus monkey blood, based on the use of a discontinuos gradient of Ficoll-Hypaque (densities, 1.100, 1.077) has been developed. Using this method, 71.1% of the PMNs were recovered in a layer with a purity of 91% PMNs with very few contaminating erythrocytes. The added advantage of the method described here is that a higher chemotactic activity was retained.

引用

页码：271 / 274

页数：4

共 9 条

[1]

AQUADO MT, 1980, J IMMUNOL METHODS, V32, P41

[2]

BOYUM A, 1968, SCAND J CLIN LAB INV, VS 21, P51

[3]

CHEUNG ATW, 1985, J MED PRIMATOL, V14, P285

[4] SINGLE-STEP SEPARATION OF RED BLOOD-CELLS, GRANULOCYTES AND MONONUCLEAR LEUKOCYTES ON DISCONTINUOUS DENSITY GRADIENTS OF FICOLL-HYPAQUE [J].