IMMUNOCHEMISTRY OF THE STREPTOCOCCUS-MUTANS BHT CELL-MEMBRANE - DETECTION OF DETERMINANTS CROSS-REACTIVE WITH HUMAN-HEART TISSUE

被引：19

作者：

AYAKAWA, GY ^{[1
]}

SIEGEL, JL ^{[1
]}

CROWLEY, PJ ^{[1
]}

BLEIWEIS, AS ^{[1
]}

机构：

[1] UNIV FLORIDA, DEPT MICROBIOL & CELL SCI, GAINESVILLE, FL 32611 USA

来源：

INFECTION AND IMMUNITY | 1985年 / 48卷 / 02期

关键词：

D O I：

10.1128/IAI.48.2.280-286.1985

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Cell membranes of S. mutans BHT serotype b were prepared after glass bead disruption of mutanolysin digestion of whole cells. Immunoblot analyses of BHT membrane extracts revealed major polypeptides of 42,000, 46,000, 62,000 and 82,000 daltons, and several minor bands, to be reactive with rabbit anti-human heart Ig. Heart cross-reactive antigens were reported in the cell walls and culture fluids of several S. mutans serotypes. This represents the 1st report of cell membrane-localized heart cross-reactive antigens in this oral pathogen. Positive enzyme-linked immunosorbent assay (ELISA)and immunoblot reactions were also obtained with heart tissue antigen and anti-BHT sera, indicating mutual cross-reactivity. The major cross-reactive component detected by immunoblotting of human extracts was a 69,000-dalton polypeptide.

引用

页码：280 / 286

页数：7

共 33 条

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