Proinflammatory Soluble Interleukin-15 Receptor Alpha Is Increased in Rheumatoid Arthritis

被引：12

作者：

Machado Diaz, Ana Cecilia ^{[1
]}

Chico Capote, Araceli ^{[2
]}

Arrieta Aguero, Celia Aurora ^{[1
]}

Rodriguez Alvarez, Yunier ^{[1
]}

del Barco Herrera, Diana Garcia ^{[1
]}

Estevez del Toro, Miguel ^{[2
]}

Guillen Nieto, Gerardo E. ^{[1
]}

Santos Savio, Alicia ^{[1
]}

机构：

[1] Ctr Genet Engn & Biotechnol, Pharmaceut Div, Havana 10600, Cuba

[2] H Ameijeiras Hosp, Rheumatol Dept, Havana 10300, Cuba

来源：

ARTHRITIS | 2012年

关键词：

D O I：

10.1155/2012/943156

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Rheumatoid arthritis (RA) is an autoimmune and inflammatory disease in which many cytokines have been implicated. In particular, IL-15 is a cytokine involved in the inflammatory processes and bone loss. The aim of this study was to investigate the existence in synovial fluid of soluble IL-15R alpha, a private receptor subunit for IL-15 which may act as an enhancer of IL-15-induced proinflammatory cytokines. Soluble IL-15R alpha was quantified by a newly developed enzyme-linked immunosorbent assay (ELISA) in samples of synovial fluid from patients with RA and osteoarthritis (OA). The levels of IL-15R alpha were significantly increased in RA patients compared to OA patients. Also, we studied the presence of membrane-bound IL-15 in cells from synovial fluids, another element necessary to induce pro-inflammatory cytokines through reverse signaling. Interestingly, we found high levels of IL-6 related to high levels of IL-15R alpha in RA but not in OA. Thus, our results evidenced presence of IL-15R alpha in synovial fluids and suggested that its pro-inflammatory effect could be related to induction of IL-6.

引用

页数：7

共 27 条

[1] RETRACTED: Reverse signaling through membrane-bound interleukin-15 (Retracted Article. See vol 286, pg 8708, 2011) [J].

Budagian, V ;

Bulanova, E ;

Orinska, Z ;

Pohl, T ;

Borden, EC ;

Silverman, R ;

Bulfone-Paus, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (40) :42192-42201

[2] A LYMPHOKINE, PROVISIONALLY DESIGNATED INTERLEUKIN-T AND PRODUCED BY A HUMAN ADULT T-CELL LEUKEMIA LINE, STIMULATES T-CELL PROLIFERATION AND THE INDUCTION OF LYMPHOKINE-ACTIVATED KILLER-CELLS [J].

BURTON, JD ;

BAMFORD, RN ;

PETERS, C ;

GRANT, AJ ;

KURYS, G ;

GOLDMAN, CK ;

BRENNAN, J ;

ROESSLER, E ;

WALDMANN, TA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (11) :4935-4939

[3] Signalling, inflammation and arthritis - Crossed signals: the role of interleukin-15 and-18 in autoimmunity [J].

Carroll, H. P. ;

Paunovic, V. ;

Gadina, M. .

RHEUMATOLOGY, 2008, 47 (09) :1269-1277

[4] Enhanced secretion of IFN-γ by activated Th1 cells occurs via reverse signaling through TNF-related activation-induced cytokine [J].

Chen, NJ ;

Huang, MW ;

Hsieh, SL .

JOURNAL OF IMMUNOLOGY, 2001, 166 (01) :270-276

[5] Inhibition of T Cell-Dependent and RANKL-Dependent Osteoclastogenic Processes Associated With High Levels of Bone Mass in Interleukin-15 Receptor-Deficient Mice [J].

Djaafar, Souad ;

Pierroz, Dominique D. ;

Chicheportiche, Rachel ;

Zheng, Xin Xiao ;

Ferrari, Serge L. ;

Ferrari-Lacraz, Sylvie .

ARTHRITIS AND RHEUMATISM, 2010, 62 (11) :3300-3310

[6] IL-15Rα recycles and presents IL-15 in trans to neighboring cells [J].

Dubois, S ;

Mariner, J ;

Waldmann, TA ;

Tagaya, Y .

IMMUNITY, 2002, 17 (05) :537-547

[7] IL-6 receptor inhibition with tocilizumab improves treatment outcomes in patients with rheumatoid arthritis refractory to anti-tumour necrosis factor biologicals: results from a 24-week multicentre randomised placebo-controlled trial [J].

Emery, P. ;

Keystone, E. ;

Tony, H. P. ;

Cantagrel, A. ;

van Vollenhoven, R. ;

Sanchez, A. ;

Alecock, E. ;

Lee, J. ;

Kremer, J. .

ANNALS OF THE RHEUMATIC DISEASES, 2008, 67 (11) :1516-1523

[8] IL-17, produced by lymphocytes and neutrophils, is necessary for lipopolysaccharide-induced airway neutrophilia: IL-15 as a possible trigger [J].

Ferretti, S ;

Bonneau, O ;

Dubois, GR ;

Jones, CE ;

Trifilieff, A .

JOURNAL OF IMMUNOLOGY, 2003, 170 (04) :2106-2112

[9] Role of trans-cellular IL-15 presentation in the activation of NK cell-mediated killing, which leads to enhanced tumor immunosurveillance [J].

Kobayashi, H ;

Dubois, S ;

Sato, N ;

Sabzevari, H ;

Sakai, Y ;

Waldmann, TA ;

Tagaya, Y .

BLOOD, 2005, 105 (02) :721-727

[10] Interleukin-6 levels in synovial fluids of patients with rheumatoid arthritis correlated with the infiltration of inflammatory cells in synovial membrane [J].

Matsumoto, Tomoko ;

Tsurumoto, Toshiyuki ;

Shindo, Hiroyuki .

RHEUMATOLOGY INTERNATIONAL, 2006, 26 (12) :1096-1100

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