EXPRESSION OF A PLASMID-ENCODED GENE FOR CADMIUM RESISTANCE OF PSEUDOMONAS-PUTIDA GAM-1 IN ESCHERICHIA-COLI

被引：3
|
作者：
KAWAI, K
HORITSU, H
HAMADA, K
ITOH, M
SUZUKI, T
TAKAMIZAWA, K
机构：
[1] Department of Biotechnology, Faculty of Agriculture, Gifu University, Gifu, 501-11, Yanagido
来源：
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1992年 / 73卷 / 04期
关键词：
D O I：
10.1016/0922-338X(92)90190-6
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A Cd2+-resistant Escherichia coli C600 transformant harboring pGU100, which was derived from Cd2+-resistant Pseudomonas putida GAM-1, was able to grow in concentrations of CdCl2 as high as 3.5 mM, whereas E. coli C600 could not grow in the presence of 1.5 mM CdCl2. E. coli C600 (pGU100) possesses a Cd2+ efflux system. This efflux system was inhibited by 100-mu-M dicyclohexylcarbodiimide, indicating that the system seems to be energy-dependent. Further studies revealed that the Cd2+ efflux system of E. coli C600 (pGU100) can operate under proliferous conditions, but not under nonproliferous conditions.
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页码：314 / 316
页数：3
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