SYSTEMIC DELIVERY OF HUMAN GROWTH-HORMONE BY INJECTION OF GENETICALLY ENGINEERED MYOBLASTS

被引：323

作者：

DHAWAN, J ^{[1
]}

PAN, LC ^{[1
]}

PAVLATH, GK ^{[1
]}

TRAVIS, MA ^{[1
]}

LANCTOT, AM ^{[1
]}

BLAU, HM ^{[1
]}

机构：

[1] STANFORD UNIV, MED CTR, SCH MED, DEPT PHARMACOL, STANFORD, CA 94305 USA

来源：

SCIENCE | 1991年 / 254卷 / 5037期

关键词：

D O I：

10.1126/science.1962213

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A recombinant gene encoding human growth hormone (hGH) was stably introduced into cultured myoblasts with a retroviral vector. After injection of genetically engineered myoblasts into mouse muscle, hGH could be detected in serum for 3 months. The fate of injected myoblasts was assessed by coinfecting the cells with two retroviral vectors, one encoding hGH and the other encoding beta-galactosidase from Escherichia coli. These results provide evidence that myoblasts, which can fuse into preexisting multinucleated myofibers that are vascularized and innervated, may be advantageous as vehicles for systemic delivery of recombinant proteins.

引用

页码：1509 / 1512

页数：4

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