CROSS-RESISTANCE TO UV-RADIATION OF A CISPLATIN-RESISTANT HUMAN CELL-LINE - OVEREXPRESSION OF CELLULAR FACTORS THAT RECOGNIZE UV-MODIFIED DNA

被引：68
作者：
CHAO, CCK
HUANG, SL
HUANG, HM
LINCHAO, S
机构：
[1] CHANG GUNG MED COLL, DEPT MED, TAOYUAN 33332, TAIWAN
[2] NATL TSING HUA UNIV, INST RADIAT BIOL, HSINCHU 30043, TAIWAN
[3] ACAD SINICA, INST MOL BIOL, TAIPEI 11529, TAIWAN
来源：
MOLECULAR AND CELLULAR BIOLOGY | 1991年 / 11卷 / 04期
关键词：
D O I：
10.1128/MCB.11.4.2075
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A human cell line selected for cisplatin resistance (CPR) was irradiated with UV light and showed cross-resistance to UV light. Applying a modified chloramphenicol acetyltransferase assay, we observed that CPR cells acquired enhanced host cell reactivation of a transfected plasmid carrying UV damage. Gel mobility shift analysis indicated that two nuclear factors that recognize UV-modified DNA were overexpressed in CPR cells. In addition, factors that bind UV-modified DNA were independent from the factors that bind cisplatin-modified DNA. The significance of the identified binding factors, possibly DNA repair enzymes, is discussed.
引用
收藏
页码：2075 / 2080
页数：6
相关论文
共 44 条
[1]  
BEHRENS BC, 1987, CANCER RES, V47, P414
[2]   TRANSLOCATION IN YEAST AND MAMMALIAN-CELLS - NOT ALL SIGNAL SEQUENCES ARE FUNCTIONALLY EQUIVALENT [J].
BIRD, P ;
GETHING, MJ ;
SAMBROOK, J .
JOURNAL OF CELL BIOLOGY, 1987, 105 (06) :2905-2914
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   AN RNA POLYMERASE-II TRANSCRIPTION FACTOR BINDS TO AN UPSTREAM ELEMENT IN THE ADENOVIRUS MAJOR LATE PROMOTER [J].
CARTHEW, RW ;
CHODOSH, LA ;
SHARP, PA .
CELL, 1985, 43 (02) :439-448
[5]  
CHAO CCK, 1991, CANCER RES, V51, P601
[6]   INDUCTION OF SISTER-CHROMATID EXCHANGES IN ICR 2A FROG CELLS EXPOSED TO 254-NM AND SOLAR UV WAVELENGTHS [J].
CHAO, CCK ;
ROSENSTEIN, BS .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1985, 41 (05) :625-627
[7]  
CHAO CS, UNPUB
[8]   XERODERMA PIGMENTOSUM GROUP-E CELLS LACK A NUCLEAR FACTOR THAT BINDS TO DAMAGED DNA [J].
CHU, G ;
CHANG, E .
SCIENCE, 1988, 242 (4878) :564-567
[9]   CISPLATIN-RESISTANT CELLS EXPRESS INCREASED LEVELS OF A FACTOR THAT RECOGNIZES DAMAGED DNA [J].
CHU, G ;
CHANG, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (09) :3324-3327
[10]   ELECTROPORATION FOR THE EFFICIENT TRANSFECTION OF MAMMALIAN-CELLS WITH DNA [J].
CHU, G ;
HAYAKAWA, H ;
BERG, P .
NUCLEIC ACIDS RESEARCH, 1987, 15 (03) :1311-1326
12345