THE SALMONELLA MAMMALIAN MICROSOME MUTAGENICITY TEST - COMPARISON OF HUMAN AND RAT LIVERS AS ACTIVATING SYSTEMS

被引：28

作者：

BEAUNE, P ^{[1
]}

LEMESTRECORNET, R ^{[1
]}

KREMERS, P ^{[1
]}

ALBERT, A ^{[1
]}

GIELEN, J ^{[1
]}

机构：

[1] UNIV LIEGE, INST PATHOL, CHIM MED LAB, B 23, B-4000 SART TILMAN, BELGIUM

来源：

MUTATION RESEARCH | 1985年 / 156卷 / 03期

关键词：

D O I：

10.1016/0165-1218(85)90056-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The mutagenicity of several test compounds was verified by the Salmonella/microsome mutagenicity test (Ames test), using both human liver and rat liver (untreated or pretreated with Aroclor 1254) S9 under identical experimental conditions. Aflatoxin B1, 3-methylcholanthrene and cigarette-smoke condensate were less mutagenic in the presence of human-liver S9 than in the presence of rat-liver S9 (particularly after treatment with Aroclor 1254). The opposite was observed with 2-aminonanthracene and to a lesser degree with 2-aminofluorene; correlation studies indicate that the 2 compounds were activated by the same or by very similar enzymes, probably cytochrome P-450. Human-liver S9, as an activating system, behaves differently than rat-liver S9; therefore, it may constitute a useful, additional tool for the study of mutagenicity and probably, carcinogenicity in man.

引用

页码：139 / 146

页数：8

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