Detection method for polygalacturonase-producing strains of Saccharomyces cerevisiae

被引：17

作者：

Gainvors, A ^{[1
]}

Belarbi, A ^{[1
]}

机构：

[1] UNIV REIMS,FAC SCI,MICROBIOL GEN & MOLEC LAB,F-51062 REIMS,FRANCE

来源：

YEAST | 1995年 / 11卷 / 15期

关键词：

Saccharomyces cerevisiae; screening; polygalacturonase; pectins;

D O I：

10.1002/yea.320111504

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the presence of glycerol or ethanol, SCPP (a strain of Saccharomyces cerevisiae that expresses pectinolytic activity) is capable of utilizing galacturonic acid or pectins for growth purposes. We now establish a relationship between the pectinolytic power of various strains of S. cerevisiae and their ability to grow on a pectin/glycerol-based medium. This property is further exploited for the detection of polygalacturonase-producing strains of S. cerevisiae.

引用

页码：1493 / 1499

页数：7

共 33 条

[11] ISOLATION AND STRUCTURE OF THE PECTIN LYASE D-ENCODING GENE FROM ASPERGILLUS-NIGER [J].

GYSLER, C ;

HARMSEN, JAM ;

KESTER, HCM ;

VISSER, J ;

HEIM, J .

GENE, 1990, 89 (01) :101-108

[12]

HASEGAWA S, 1962, J BIOL CHEM, V234, P619

[13]

KILGORE WW, 1959, J BIOL CHEM, V234, P2227

[14] SYNTHESIS AND SECRETION OF AN ERWINIA-CHRYSANTHEMI PECTATE LYASE IN SACCHAROMYCES-CEREVISIAE REGULATED BY DIFFERENT COMBINATIONS OF BACTERIAL AND YEAST PROMOTER AND SIGNAL SEQUENCES [J].

LAING, E ;

PRETORIUS, IS .

GENE, 1992, 121 (01) :35-45

[15] SIMULTANEOUS MEASUREMENT OF THE GALACTURONATE AND NEUTRAL SUGAR CONTENTS OF PECTIC SUBSTANCES BY AN ENZYMATIC-HPLC METHOD [J].

MATSUHASHI, S ;

INOUE, S ;

HATANAKA, C .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1992, 56 (07) :1053-1057

[16] COLORIMETRIC DETERMINATION OF PECTIC SUBSTANCES [J].

MCCOMB, EA ;

MCCREADY, RM .

ANALYTICAL CHEMISTRY, 1952, 24 (10) :1630-1632

[17] A PLATE ASSAY-METHOD FOR THE DETECTION OF FUNGAL POLYGALACTURONASE SECRETION [J].

MCKAY, AM .

FEMS MICROBIOLOGY LETTERS, 1988, 56 (03) :355-358

[18] DEGRADATION OF POLYGALACTURONIC ACID BY SACCHAROMYCES-CEREVISIAE [J].

MCKAY, AM .

LETTERS IN APPLIED MICROBIOLOGY, 1990, 11 (01) :41-44

[19] USE OF DINITROSALICYLIC ACID REAGENT FOR DETERMINATION OF REDUCING SUGAR [J].

MILLER, GL .

ANALYTICAL CHEMISTRY, 1959, 31 (03) :426-428

[20]

Milner Y., 1967, CARBOHYD RES, V4, P359, DOI DOI 10.1016/S0008-6215(00)80191-3

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