DISTINCT EFFECTS OF THROMBOSPONDIN-1 AND CISP THROMBOSPONDIN-2 ON ADRENOCORTICAL CELL SPREADING

被引：19

作者：

PELLERIN, S ^{[1
]}

LAFEUILLADE, B ^{[1
]}

CHAMBAZ, EM ^{[1
]}

FEIGE, JJ ^{[1
]}

机构：

[1] CEN, DEPT BIOL MOLEC & STRUCT, CEA, INSERM, U244, F-38054 GRENOBLE 9, FRANCE

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1994年 / 106卷 / 1-2期

关键词：

THROMBOSPONDIN; CORTICOTROPIN-INDUCED SECRETED PROTEIN; ADRENAL CORTEX; CELL ADHESION; CELL SPREADING;

D O I：

10.1016/0303-7207(94)90201-1

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Corticotropin-induced secreted protein (CISP) is a trimeric protein secreted by bovine adrenocortical cells in response to ACTH, that is likely to represent the bovine form of thrombospondin-2 (TSP2). This study was aimed at delineating the respective effects of CISP/TSP2 and TSP1 (thrombospondin-1) on adrenocortical cell attachment and spreading. TSP1 and CISP/TSP2 were found to slightly reduce the attachment of adrenocortical cells to plastic in the presence of serum but exhibited a pronounced differential effect on cell spreading. CISP/TSP2 inhibited adrenocortical cell spreading in a dose-dependent manner (maximal effect with 40 mu g/ml) whereas TSP1 (up to 100 mu g/ml) did not influence this process. The inhibition of spreading was observed whether plates were coated with CISP/TSP alone or with a mixture of CISP/TSP2 and fibronectin. We suggest that the inhibition of in vitro adrenocortical cell spreading by CISP/TSP2 is indicative of an implication of this protein in the migration of adrenocortical cells in vivo.

引用

页码：181 / 186

页数：6

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