CONDITIONAL GROWTH OF ESCHERICHIA-COLI CAUSED BY EXPRESSION OF VACCINIA VIRUS-DNA TOPOISOMERASE-I

被引：13

作者：

FERNANDEZBEROS, ME ^{[1
]}

TSEDINH, YC ^{[1
]}

机构：

[1] NEW YORK MED COLL,DEPT BIOCHEM & MOLEC BIOL,VALHALLA,NY 10595

来源：

JOURNAL OF BACTERIOLOGY | 1992年 / 174卷 / 21期

关键词：

D O I：

10.1128/JB.174.21.7059-7062.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Active vaccinia virus topoisomerase I is expressed in Escherichia coli containing plasmid p1940 (S. Shuman, M. Golder, and B. Moss, J. Biol. Chem. 263:16401-16407, 1988). Growth curves showed a decline of 2 to 3 logs in the number of viable cells at 42-degrees-C after shift from 30-degrees-C because of increased vaccinia virus topoisomerase I level. Mutations in the gyrA and gyrB genes allowed cells to grow equally well at 42 and 30-degrees-C. The presence of gyrase inhibitor also improved growth at 42-degrees-C.

引用

页码：7059 / 7062

页数：4

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