Optimal Timing for Oocyte Denudation and Intracytoplasmic Sperm Injection

被引：31

作者：

Patrat, Catherine ^{[1
]}

Kaffel, Aida ^{[2
]}

Delaroche, Lucie ^{[1
]}

Guibert, Juliette ^{[3
]}

Jouannet, Pierre ^{[2
]}

Epelboin, Sylvie ^{[4
]}

De Ziegler, Dominique ^{[5
]}

Wolf, Jean-Philippe ^{[2
]}

Fauque, Patricia ^{[6
,7
]}

机构：

[1] Univ Paris Diderot, AP HP, Hop Bichat, Reprod Biol Lab, F-75018 Paris, France

[2] AP HP, Hop Cochin, Reprod Biol Lab, F-75014 Paris, France

[3] Inst Mutualiste Monstsouris, Serv Gynecol Obstet, F-75014 Paris, France

[4] AP HP, Hop Bichat, Serv Gynecol Obstet 2, F-75018 Paris, France

[5] Univ Paris 05, AP HP, Hop Cochin, Serv Gynecol Obstet 2, F-75005 Paris, France

[6] Univ Bourgogne, CHU Dijon, Lab Biol Reprod CECOS, Dijon, France

[7] CNRS, INSERM U934, Inst Curie, UMR 3215, Paris, France

来源：

OBSTETRICS AND GYNECOLOGY INTERNATIONAL | 2012年 / 2012卷

关键词：

D O I：

10.1155/2012/403531

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objectives. To analyze the impact of oocyte denudation and microinjection timings on intracytoplasmic sperm injection ( ICSI) outcomes. Study Design. We included ICSI cycles with the following parameters: rank 1 or 2, female age < 36 years, male factor infertility, long protocol using GnRH agonist and rFSH for ovarian stimulation, and use of freshly ejaculated sperm (n = 110). Several ICSI parameters were analyzed according to the time between oocyte retrieval and denudation (T-1) and the time between denudation and ICSI (T-2) using a statistical logistic regression analysis. Results. Neither T-1 nor T-2 had a significant influence on the Metaphase II (MII) rate but the fertilisation rate (FR) showed a significant improvement when T1 was longer (optimal results at T-1 = 3 hours) while FR significantly decreased with the increase of T-2. Optimal implantation (IR) and pregnancy (PR) rates were obtained when T-1 was around 2 hours. Conclusion. Incubation of oocytes around 2 hours between retrieval and denudation may not increase MII rate but appears to lead to the optimal combination of FR and IR.

引用

页数：7

共 26 条

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