CLONING AND SEQUENCING OF PEPC, A CYSTEINE AMINOPEPTIDASE GENE FROM LACTOCOCCUS-LACTIS SUBSP CREMORIS AM2

被引：56
|
作者：
CHAPOTCHARTIER, MP [1 ]
NARDI, M [1 ]
CHOPIN, MC [1 ]
CHOPIN, A [1 ]
GRIPON, JC [1 ]
机构：
[1] INRA,GENET MICROBIENNE LAB,F-78352 JOUY EN JOSAS,FRANCE
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 01期
关键词：
D O I：
10.1128/AEM.59.1.330-333.1993
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A gene coding for an aminopeptidase (PepC) from Lactococcus lactis subsp. cremoris AM2 was cloned by complementation of an Escherichia coli mutant lacking aminopeptidase activity. The nucleotide sequence was determined. A portion of the predicted amino acid sequence of PepC (436 amino acids) showed strong homology to the active site of cysteine proteases. No signal sequence was found, indicating an intracellular location of the enzyme.
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页码：330 / 333
页数：4
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