ESTABLISHMENT OF SYNCHRONY BY STARVATION AND READDITION OF AUXIN IN SUSPENSION-CULTURES OF CATHARANTHUS-ROSEUS CELLS

被引：27

作者：

NISHIDA, T ^{[1
]}

OHNISHI, N ^{[1
]}

KODAMA, H ^{[1
]}

KOMAMINE, A ^{[1
]}

机构：

[1] TOHOKU UNIV,FAC SCI,INST BIOL,SENDAI,MIYAGI 980,JAPAN

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1992年 / 28卷 / 01期

关键词：

AUXIN STARVATION; CATHARANTHUS-ROSEUS; CELL CYCLE; PERIWINKLE; SYNCHRONOUS CULTURE;

D O I：

10.1007/BF00039913

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A system of synchronous cell division was established by starvation of auxin and its readdition to suspension cultures of cells of Catharanthus roseus L. cv. Little-Pinky. When cells in the stationary phase were transferred to fresh medium free of 2,4-dichlorophenoxyacetic acid (2,4-D), cells were arrested preferentially at the G1 phase. After cells had been cultured for 2 days in medium without 2,4-D, readdition of 2,4-D induced the synchronous division of cells. In this system, 70-80% of cells divided synchronously within 3 to 4h, and the mitotic index increased sharply in parallel with the increase in cell number. Active synthesis of DNA was demonstrated by measurements of incorporation of [H-3]-thymidine into the DNA fraction. The induction of cell division by the addition of 2,4-D) inhibited by treating cells with analogues of auxin, such as 2,4,6-trichlorophenoxyacetic acid and p-chlorophenoxyisobutyric acid.

引用

页码：37 / 43

页数：7

共 19 条

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