A 2-STEP ENZYMATIC-SYNTHESIS OF DIPEPTIDES

被引：21
作者：
SCHWARZ, A
WANDREY, C
STEINKE, D
KULA, MR
机构：
[1] FORSCHUNGSZENTRUM JULICH, RES CTR, INST BIOTECHNOL, W-5170 JULICH 1, GERMANY
[2] UNIV DUSSELDORF, RES CTR JULICH, INST ENZYME TECHNOL, W-5170 JULICH, GERMANY
来源：
BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 39卷 / 02期
关键词：
ENZYMATIC SYNTHESIS; CONTINUOUS PRODUCTION; ANION-EXCHANGE; PEPTIDE-AMIDASE;
D O I：
10.1002/bit.260390203
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A simple system is introduced to produce dipeptides continuously by enzyme catalyzed condensation of amino acid esters and amino acid amides. Synthesis of N-terminal free dipeptide-amides is achieved by means of carboxypeptidase Y. The peptide-amide is deamidated utilizing a newly isolated peptide-amidase. Separation of substrates and products is accomplished by anion-exchange chromatography. Modeling of the reactions shows that the two reactions have to be carried out in a cascade of two reactors in order to prevent hydrolysis of the peptide by the carboxypeptidase. Continuous production of Kyotorphin (H-TyrArg-OH) with a space-time yield of 257 g/L d shows the feasibility of this concept.
引用
收藏
页码：132 / 140
页数：9
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