PROTEIN ADSORPTION ON THE SURFACE OF A THIN-FILM POLYMER INTEGRATED OPTICAL WAVE-GUIDE

被引:23
作者
IVES, JT [1 ]
REICHERT, WM [1 ]
机构
[1] UNIV UTAH,CTR SENSOR TECHNOL,SALT LAKE CITY,UT 84112
关键词
FLUORESCEIN ISOTHIOCYANATE - INTEGRATED OPTICAL WAVEGUIDE - MONOLAYER STRUCTURE;
D O I
10.1366/0003702884428572
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
Protein adsorption (fluorescein labeled gamma globulin, FITC-IgG) on the surface of poly(styrene) thin-film optical waveguides was fluorescently detected. The time course of adsorption, the FITC-IgG fluorescence spectra, and the surface concentration estimates are presented. The results demonstrate the potential use of polymer optical waveguides as integrated optic evanescent sensors.
引用
收藏
页码:68 / 72
页数:5
相关论文
共 12 条
[1]   THE ADSORPTIVE CHARACTERISTICS OF PROTEINS FOR POLYSTYRENE AND THEIR SIGNIFICANCE IN SOLID-PHASE IMMUNOASSAYS [J].
CANTARERO, LA ;
BUTLER, JE ;
OSBORNE, JW .
ANALYTICAL BIOCHEMISTRY, 1980, 105 (02) :375-382
[2]   FLUORESCENCE OF ADSORBED PROTEIN LAYERS .1. QUANTITATION OF TOTAL INTERNAL-REFLECTION FLUORESCENCE [J].
HLADY, V ;
REINECKE, DR ;
ANDRADE, JD .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1986, 111 (02) :555-569
[3]  
Lakowicz JR., 2006, PRINCIPLES FLUORESCE, V3
[4]  
Marcuse D., 1974, THEORY DIELECTRIC OP
[5]   QUANTITATIVE RAMAN-SPECTROSCOPY OF HOMOGENEOUS MOLECULAR PROFILES IN OPTICAL WAVE-GUIDES USING INTERNAL STANDARDS [J].
MILLER, DR ;
HAN, OH ;
BOHN, PW .
APPLIED SPECTROSCOPY, 1987, 41 (02) :249-255
[6]   REMOTE SPECTROSCOPIC SENSING OF CHEMICAL ADSORPTION USING A SINGLE MULTIMODE OPTICAL FIBER [J].
NEWBY, K ;
REICHERT, WM ;
ANDRADE, JD ;
BENNER, RE .
APPLIED OPTICS, 1984, 23 (11) :1812-1815
[7]   INTEGRATED-OPTICS AND RAMAN-SCATTERING - MOLECULAR-ORIENTATION IN THIN POLYMER-FILMS AND LANGMUIR-BLODGETT MONOLAYERS [J].
RABOLT, JF ;
SANTO, R ;
SCHLOTTER, NE ;
SWALEN, JD .
IBM JOURNAL OF RESEARCH AND DEVELOPMENT, 1982, 26 (02) :209-216
[8]  
RABOLT JF, 1983, J POLYM SCI, V12, P1
[9]   EXCITATION OF FLUORESCENT EMISSION FROM SOLUTIONS AT THE SURFACE OF POLYMER THIN-FILM WAVE-GUIDES - AN INTEGRATED-OPTICS TECHNIQUE FOR THE SENSING OF FLUORESCENCE AT THE POLYMER-SOLUTION INTERFACE [J].
REICHERT, WM ;
IVES, JT ;
SUCI, PA ;
HLADY, V .
APPLIED SPECTROSCOPY, 1987, 41 (04) :636-640
[10]  
REICHERT WM, 1985, T ANN M SOC BIOMAT S