MUTAGENESIS AND SELF-LIGATION OF THE SELF-CLEAVAGE DOMAIN OF THE SATELLITE RNA MINUS STRAND OF TOBACCO RINGSPOT VIRUS AND ITS BINDING TO POLYAMINES

被引：18

作者：

SEKIGUCHI, A ^{[1
]}

KOMATSU, Y ^{[1
]}

KOIZUMI, M ^{[1
]}

OHTSUKA, E ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,SAPPORO,HOKKAIDO 060,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 24期

关键词：

D O I：

10.1093/nar/19.24.6833

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several mutants for the minus strands of the self-cleaving domain of the satellite RNA of tobacco ringspot virus have been synthesized by joining chemically synthesized oligoribonucleotides with RNA ligase. Kinetic properties of the enzyme strands (50 nucleotides) against substrates (15-mer and 18-mer) were investigated. Structural properties of the unpaired part in the cleavage region were estimated from mutagenesis. The catalytic domain alone was proved to be responsible for the rejoining reaction of cleaved substrates. It was also found that the ribozyme could be divided into two strands without loss of activity. Effects of concentration of magnesium ion and polyamines on the cleavage reaction for the two-stranded ribozyme are also reported.

引用

页码：6833 / 6838

页数：6

共 7 条

[1] MUTAGENESIS ANALYSIS OF THE SELF-CLEAVAGE DOMAIN OF HEPATITIS DELTA VIRUS ANTIGENOMIC RNA
WU, HN
HUANG, ZS
NUCLEIC ACIDS RESEARCH, 1992, 20 (22) : 5937 - 5941
[2] SEQUENCES REQUIRED FOR SELF-CATALYZED CLEAVAGE OF THE SATELLITE RNA OF TOBACCO RINGSPOT VIRUS
HASELOFF, J
GERLACH, WL
GENE, 1989, 82 (01) : 43 - 52
[3] A SATELLITE RNA OF BARLEY YELLOW DWARF VIRUS CONTAINS A NOVEL HAMMERHEAD STRUCTURE IN THE SELF-CLEAVAGE DOMAIN
MILLER, WA
HERCUS, T
WATERHOUSE, PM
GERLACH, WL
VIROLOGY, 1991, 183 (02) : 711 - 720
[4] ALTERNATIVE TERTIARY STRUCTURE ATTENUATES SELF-CLEAVAGE OF THE RIBOZYME IN THE SATELLITE RNA OF BARLEY YELLOW DWARF VIRUS
MILLER, WA
SILVER, SL
NUCLEIC ACIDS RESEARCH, 1991, 19 (19) : 5313 - 5320
[5] EXPRESSION OF HEPATITIS-DELTA VIRUS-RNA DELETIONS - CIS AND TRANS REQUIREMENTS FOR SELF-CLEAVAGE, LIGATION, AND RNA PACKAGING
LAZINSKI, DW
TAYLOR, JM
JOURNAL OF VIROLOGY, 1994, 68 (05) : 2879 - 2888
[6] SELF-CLEAVAGE OF HEPATITIS DELTA-VIRUS GENOMIC STRAND RNA IS ENHANCED UNDER PARTIALLY DENATURING CONDITIONS
ROSENSTEIN, SP
BEEN, MD
BIOCHEMISTRY, 1990, 29 (35) : 8011 - 8016
[7] Satellite cereal yellow dwarf virus-RPV (satRPV) RNA requires a DouXble hammerhead for self-cleavage and an alternative structure for replication
Song, SI
Silver, SL
Aulik, MA
Rasochova, L
Mohan, BR
Miller, WA
JOURNAL OF MOLECULAR BIOLOGY, 1999, 293 (04) : 781 - 793

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