PARALLEL ANALYSIS OF OLIGODEOXYRIBONUCLEOTIDE (OLIGONUCLEOTIDE) INTERACTIONS .1. ANALYSIS OF FACTORS INFLUENCING OLIGONUCLEOTIDE DUPLEX FORMATION

被引：94
作者：
MASKOS, U
SOUTHERN, EM
机构：
[1] Department of Biochemistry, University of Oxford, Oxford OX1 3QU, South Parks Road
来源：
NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 07期
关键词：
D O I：
10.1093/nar/20.7.1675
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel method for the analysis of oligonucleotide - oligonucleotide interactions is described. Oligonucleotides of different sequence are synthesised in situ as stripes on the surface of a glass slide (see accompanying paper). Multiple hybridisations are then carried out on each oligonucleotide simultaneously to determine the dependence of oligonucleotide duplex formation on duplex length, base composition, hybridisation solvent and sequence complexity.
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页码：1675 / 1678
页数：4
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