ANTHRAX PROTECTIVE ANTIGEN INTERACTS WITH A SPECIFIC RECEPTOR ON THE SURFACE OF CHO-K1 CELLS

被引:107
作者
ESCUYER, V
COLLIER, RJ
机构
[1] HARVARD UNIV, SCH MED, DEPT MICROBIOL & MOLEC GENET, 200 LONGWOOD AVE, BOSTON, MA 02115 USA
[2] HARVARD UNIV, SCH MED, SHIPLEY INST MED, BOSTON, MA 02115 USA
关键词
D O I
10.1128/IAI.59.10.3381-3386.1991
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The interaction of protective antigen (PA), a component of the anthrax toxin, with receptors on the Chinese hamster ovary cell line CHO-K1 was characterized. Protective antigen binding at 4-degrees-C is highly specific, concentration dependent, saturable (K(d) = 0.9 nM), and reversible. Scatchard analysis indicates the presence of a single class of PA binding sites at a concentration of 10,000 +/- 2,000 per cell. Pretreatment of cells with a number of different proteases strongly inhibits PA binding, suggesting that the receptor may be at least partially proteinaceous. Direct chemical cross-linking of radioiodinated PA to the cell surface results in the appearance of a major band exhibiting an apparent molecular mass of 170 kDa, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The appearance of this band is completely inhibited by a 200-fold molar excess of unlabeled PA, indicating a high specificity for this interaction. Our results suggest that a cell surface protein(s) of 85 to 90 kDa is, or constitutes a portion of, a specific receptor for the PA.
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收藏
页码:3381 / 3386
页数:6
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