SECRETOGRANIN-II IS SYNTHESIZED AND SECRETED IN ASTROCYTE CULTURES

被引:22
作者
FISCHERCOLBRIE, R
KIRCHMAIR, R
SCHOBERT, A
OLENIK, C
MEYER, DK
WINKLER, H
机构
[1] UNIV INNSBRUCK, DEPT PHARMACOL, PETER MAYR STR 1A, A-6020 INNSBRUCK, AUSTRIA
[2] UNIV FREIBURG, DEPT PHARMACOL, W-7800 FREIBURG, GERMANY
关键词
CHROMOGRANINS; CARBOXYPEPTIDASE-H; SECRETONEURIN;
D O I
10.1111/j.1471-4159.1993.tb03520.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Astrocyte cultures from rat brain were analyzed for their ability to synthesize and secrete secretogranin II (chromogranin C). Northern blot analysis of polyA-selected RNA established the presence of secretogranin II mRNA in these cells. By radioimmunoassay, 11.6 fmol/10(6) astrocytes of secretogranin II was found in these cells. About twice the amount was released into the medium within 3 days. Secretogranin II within the astrocytes was practically unprocessed, as shown by HPLC. These results establish for the first time that astrocytes in vitro synthesize and secrete a protein of the acidic chromogranin family.
引用
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页码:2312 / 2314
页数:3
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