SECRETOGRANIN-II IS SYNTHESIZED AND SECRETED IN ASTROCYTE CULTURES

被引：22

作者：

FISCHERCOLBRIE, R

KIRCHMAIR, R

SCHOBERT, A

OLENIK, C

MEYER, DK

WINKLER, H

机构：

[1] UNIV INNSBRUCK, DEPT PHARMACOL, PETER MAYR STR 1A, A-6020 INNSBRUCK, AUSTRIA

[2] UNIV FREIBURG, DEPT PHARMACOL, W-7800 FREIBURG, GERMANY

来源：

JOURNAL OF NEUROCHEMISTRY | 1993年 / 60卷 / 06期

关键词：

CHROMOGRANINS; CARBOXYPEPTIDASE-H; SECRETONEURIN;

D O I：

10.1111/j.1471-4159.1993.tb03520.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Astrocyte cultures from rat brain were analyzed for their ability to synthesize and secrete secretogranin II (chromogranin C). Northern blot analysis of polyA-selected RNA established the presence of secretogranin II mRNA in these cells. By radioimmunoassay, 11.6 fmol/10(6) astrocytes of secretogranin II was found in these cells. About twice the amount was released into the medium within 3 days. Secretogranin II within the astrocytes was practically unprocessed, as shown by HPLC. These results establish for the first time that astrocytes in vitro synthesize and secrete a protein of the acidic chromogranin family.

引用

页码：2312 / 2314

页数：3

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