The aim of this study was to investigate: a) the influence of perceived constraints on recreational sport participation of Greek university students, b) the relationship between constraints dimensions and sport participation or non participation; and c) the relationship between constraint dimensions and frequency of sport participation, as well as intentions for participation. The methodology employed included review of relevant literature and administration of a specifically designed questionnaire, the University Sport Constraints Questionnaire (USCQ). The sample consisted of 3,041 students from 7 Greek universities. The results showed that there were 9 factors which explained 69.75% of overall variance. The most important constraint that affects participation was found to be "Accessibility," followed by "Lack of Knowledge." It was concluded that to increase the students' participation rate, campus recreation administrators need to develop effective strategies to design and promote campus sports and recreational programs.