MULTIPLE CELL-CYCLE ACCESS TO THE APOPTOTIC DEATH PROGRAM IN HUMAN NEUROBLASTOMA-CELLS

被引：73
作者：
PIACENTINI, M
FESUS, L
MELINO, G
机构：
[1] UNIV ROMA TOR VERGATA,DIPARTIMENTO MED SPERIMENTALE & SCI BIOCHIM,VIA O RAIMONDO,I-00173 ROME,ITALY
[2] UNIV ROMA TOR VERGATA,DEPT BIOL,ROME,ITALY
[3] LAJOS KOSSUTH UNIV,SCH MED,DEPT BIOCHEM,H-4010 DEBRECEN,HUNGARY
来源：
FEBS LETTERS | 1993年 / 320卷 / 02期
关键词：
APOPTOSIS; NEUROBLASTOMA; CISPLATIN; TISSUE TRANSGLUTAMINASE;
D O I：
10.1016/0014-5793(93)80081-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the induction of apoptosis in a human neuroblastoma cell line SK-N-BE(2) by cisplatin or retinoic acid, and its relation to cell cycle. Apoptosis was monitored by counting apoptotic bodies and evaluating the activity of 'tissue' transglutaminase (EC 2.3.2.13), one of the genes specifically expressed in apoptotic cells. Data indicate that both agents enhance apoptosis, even though cells arrest at different cell cycle phases. In fact, retinoic acid causes accumulation in G1, whilst cisplatin induces accumulation of cells in the G2/M phase. This evidence suggests the presence of multiple start points for the apoptotic death programme within the cell cycle of human neuroblastoma cells.
引用
收藏
页码：150 / 154
页数：5
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