STRUCTURE AND EXPRESSION OF A GENE FROM ARABIDOPSIS-THALIANA ENCODING A PROTEIN RELATED TO SNF1 PROTEIN-KINASE

被引：1

作者：

LEGUEN, L ^{[1
]}

THOMAS, M ^{[1
]}

BIANCHI, M ^{[1
]}

HALFORD, NG ^{[1
]}

KREIS, M ^{[1
]}

机构：

[1] UNIV BRISTOL,LONG ASHTON RES STN,BRISTOL BS18 9AF,AVON,ENGLAND

来源：

GENE | 1992年 / 120卷 / 02期

关键词：

RECOMBINANT DNA; GENE EXPRESSION; INTRONS; EXONS;

D O I：

暂无

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The AKin10 gene from Arabidopsis thaliana encoding a putative Ser/Thr protein kinase (PK) has been isolated and characterized. The AKin10-encoding gene is located on a genomic 5.4-kb BamHI fragment and contains ten introns, one being located in the 5' untranslated region. The deduced amino acid sequence of AKin10 is 65% identical over the catalytic domain to the yeast PK (SNF1). SNF1 is essential for the derepression of many glucose-repressible genes, including Suc2 which encodes invertase. Southern blot hybridization experiments suggested the presence of one copy of the gene per haploid genome of A. thaliana. Northern hybridization experiments indicated that this gene is expressed in roots, shoots and leaves. AKin10 may play an important role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.

引用

页码：249 / 254

页数：6

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