Using a microorganism consortium for consolidated bioprocessing cellulosic ethanol production

A facultative 'consortium H', capable of degrading cellulose and producing cellulosic ethanol by consolidated bioprocessing, with an ethanol concentration of 1.54 g/l and 5 g filter paper being used as carbon resource, was obtained through restrictive subculture. Based on the control experiments, it is believed that most ethanol was produced from the carbon source, which meant that the conversion ratio from cellulose to ethanol was approximately 60%. PCR-denaturing gradient gel electrophoresis and thermal desorption-mass spectrometry technology were used to analyze the component strains of the consortium. The data from the genomics and proteomics study showed that Clostridia bacteria were the dominant strains. This could potentially be used to increase the cellulose conversion ratio and improve ethanol production by optimizing and reconstructing the consortium.