HYPOXIA-INDUCIBLE NUCLEAR FACTORS BIND TO AN ENHANCER ELEMENT LOCATED 3' TO THE HUMAN ERYTHROPOIETIN GENE

被引：763

作者：

SEMENZA, GL ^{[1
]}

NEJFELT, MK ^{[1
]}

CHI, SM ^{[1
]}

ANTONARAKIS, SE ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV, SCH MED, DEPT MED, BALTIMORE, MD 21205 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 13期

关键词：

D O I：

10.1073/pnas.88.13.5680

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Human erythropoietin gene expression in liver and kidney is inducible by anemia or hypoxia. DNase I-hypersensitive sites were identified 3' to the human erythropoietin gene in liver nuclei. A 256-base-pair region of 3' flanking sequence was shown by DNase I protection and electrophoretic mobility-shift assays to bind four or more different nuclear factors, at least two of which are induced by anemia in both liver and kidney, and the region functioned as a hypoxia-inducible enhancer in transient expression assays. These results provide insight into the molecular basis for the regulation of gene expression by a fundamental physiologic stimulus, hypoxia.

引用

页码：5680 / 5684

页数：5

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