Spheroid array of fetal mouse liver cells constructed on a PEG-gel micropatterned surface: upregulation of hepatic functions by co-culture with nonparenchymal liver cells

被引：27

作者：

Kojima, Ryota ^{[1
]}

Yoshimoto, Keitaro ^{[1
,2
,3
]}

Takahashi, Emiko ^{[1
]}

Ichino, Masahiro ^{[1
]}

Miyoshi, Hirotoshi ^{[4
,5
]}

Nagasaki, Yukio ^{[1
,2
,3
,4
,6
]}

机构：

[1] Univ Tsukuba, Grad Sch Pure & Appl Sci, Tsukuba, Ibaraki 3058573, Japan

[2] Univ Tsukuba, Ctr Tsukuba Adv Res Alliance, Tsukuba, Ibaraki 3058577, Japan

[3] Univ Tsukuba, Tsukuba Res Ctr Interdisciplinary Mat Sci, Tsukuba, Ibaraki 3058571, Japan

[4] Univ Tsukuba, Grad Sch Comprehens Human Sci, Masters Sch Med Sci, Tsukuba, Ibaraki 3058575, Japan

[5] Univ Tsukuba, Grad Sch Comprehens Human Sci, Dept Biomed Engn, Tsukuba, Ibaraki 3058575, Japan

[6] NIMS, Int Ctr Mat Nanoarchitecton MANA, Satellite Lab, Tsukuba, Ibaraki 3058573, Japan

来源：

LAB ON A CHIP | 2009年 / 9卷 / 14期

关键词：

ONCOSTATIN-M; HEPATOCYTES; MATURATION; RAT;

D O I：

10.1039/b903388b

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A spheroid array of fetal mouse liver cells, which comprise various immature cells, was constructed on a PEG-gel micropatterned surface and its hepatic activity and degree of differentiation induction were significantly upregulated by co-culture with nonparenchymal liver cells as feeder-cells.

引用

页码：1991 / 1993

页数：3

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