CONCENTRATION-EFFECT IN HYALURONAN ANALYSIS BY SIZE-EXCLUSION CHROMATOGRAPHY

被引：7
|
作者：
ORVISKY, E
SOLTES, L
ALASSAF, S
机构：
[1] SLOVAK ACAD SCI, INST EXPTL PHARMACOL, BRATISLAVA 84216, SLOVAKIA
[2] NE WALES INST, FAC SCI HLTH & MED STUDIES, DIV RES, DEESIDE CH5 4BR, CLWYD, WALES
来源：
CHROMATOGRAPHIA | 1994年 / 39卷 / 5-6期
关键词：
SIZE-EXCLUSION CHROMATOGRAPHY; CONCENTRATION EFFECTS; HYALURONAN; HYALURONATE;
D O I：
10.1007/BF02274527
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The concentration effect in size-exclusion chromatographic analysis of hyaluronans of various relative molecular masses (RMM) has been studied. A critical concentration has been found that is negatively dependent on the hyaluronan molecular mass; the higher the biopolymer molecular mass, the smaller the injected sample where the concentration effect should be taken into account for accurate evaluation of molecular mass distribution. At higher temperatures, however, the concentration effect diminishes significantly.
引用
收藏
页码：366 / 368
页数：3
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