MULTISITE REPRODUCIBILITY OF COLORIMETRIC BROTH MICRODILUTION METHOD FOR ANTIFUNGAL SUSCEPTIBILITY TESTING OF YEAST ISOLATES

被引：56
作者：
PFALLER, MA
VU, Q
LANCASTER, M
ESPINELINGROFF, A
FOTHERGILL, A
GRANT, C
MCGINNIS, MR
PASARELL, L
RINALDI, MG
STEELEMOORE, L
机构：
[1] OREGON HLTH SCI UNIV,PORTLAND,OR 97201
[2] ALAMAR BIOSCI INC,SACRAMENTO,CA
[3] VIRGINIA COMMONWEALTH UNIV MED COLL VIRGINIA,RICHMOND,VA
[4] UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX
[5] UNIV TEXAS,MED BRANCH,GALVESTON,TX 77550
[6] CASE WESTERN RESERVE UNIV,CLEVELAND,OH 44106
[7] MED CTR DELAWARE,WILMINGTON,DE
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1994年 / 32卷 / 07期
关键词：
D O I：
10.1128/JCM.32.7.1625-1628.1994
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode +/- 1 log(2) dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.6% with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.
引用
收藏
页码：1625 / 1628
页数：4
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