MULTISITE REPRODUCIBILITY OF COLORIMETRIC BROTH MICRODILUTION METHOD FOR ANTIFUNGAL SUSCEPTIBILITY TESTING OF YEAST ISOLATES

被引：56

作者：

PFALLER, MA

VU, Q

LANCASTER, M

ESPINELINGROFF, A

FOTHERGILL, A

GRANT, C

MCGINNIS, MR

PASARELL, L

RINALDI, MG

STEELEMOORE, L

机构：

[1] OREGON HLTH SCI UNIV,PORTLAND,OR 97201

[2] ALAMAR BIOSCI INC,SACRAMENTO,CA

[3] VIRGINIA COMMONWEALTH UNIV MED COLL VIRGINIA,RICHMOND,VA

[4] UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX

[5] UNIV TEXAS,MED BRANCH,GALVESTON,TX 77550

[6] CASE WESTERN RESERVE UNIV,CLEVELAND,OH 44106

[7] MED CTR DELAWARE,WILMINGTON,DE

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1994年 / 32卷 / 07期

关键词：

D O I：

10.1128/JCM.32.7.1625-1628.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode +/- 1 log(2) dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.6% with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.

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收藏

页码：1625 / 1628

页数：4

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