ANALYSIS OF SNAKE-VENOMS BY SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS AND 2-DIMENSIONAL ELECTROPHORESIS

被引：1

作者：

MARSHALL, T

WILLIAMS, KM

机构：

[1] School of Health Sciences, The University of Sunderland, Sunderland, SR2 7EE, Fleming Building

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 662卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)85306-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The protein composition of snake venom has been analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and high-resolution two-dimensional electrophoresis (2-DE). SDS-PAGE suggests differences between species and similarities between related species. As expected, silver staining greatly enhanced detection sensitivity whilst 2-DE dramatically improved separation to reveal multiple strings of spots indicative of molecular heterogeneity. It is recommended that similarities (or differences) in the SDS-PAGE patterns of venoms from the same (or different) species of snake should be further characterized by 2-DE.

引用

页码：167 / 172

页数：6

共 15 条

[1] ANTIGENIC RELATIONSHIPS AND RELATIVE IMMUNOGENICITIES OF VENOM PROTEINS FROM 6 POISONOUS SNAKES OF THAILAND [J].