GENETIC-TRANSFORMATION OF ASPEN (POPULUS-TREMULAXPOPULUS-ALBA) BY AGROBACTERIUM-RHIZOGENES AND REGENERATION OF PLANTS TOLERANT TO HERBICIDE

被引：0
|
作者：
DEVILLARD, C [1 ]
机构：
[1] ASSOC FORET CELLULOSE,BIOTECHNOL STN,F-77370 NANGIS,FRANCE
来源：
COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE III-SCIENCES DE LA VIE-LIFE SCIENCES | 1992年 / 314卷 / 06期
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D O I：
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中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
An oncogenic strain of Agrobacterium rhizogenes, containing the bar gene which confers resistance to the herbicide Basta, was used for transformation of an aspen hybrid (Populus tremula x Populus alba). From 28 to 54% of inoculated stem sections formed roots after inoculation by the bacteria according to methods of inoculation. Plants were regenerated from transformed roots. Successful genetic transformation was confirmed by enzyme assays and Southern blot analysis. Transgenic plants transferred to soil and sprayed in the greenhouse showed tolerance towards Basta.
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页码：291 / 298
页数：8
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