FLUORESCEIN AND FLUORESCEIN GLUCURONIDE IN PLASMA

被引：0

作者：

KNUDSEN, LL ^{[1
]}

OLSEN, T ^{[1
]}

NIELSENKUDSK, F ^{[1
]}

机构：

[1] AARHUS UNIV,INST PHARMACOL,DK-8000 AARHUS,DENMARK

来源：

ACTA OPHTHALMOLOGICA | 1992年 / 70卷 / 04期

关键词：

VITREOUS FLUOROPHOTOMETRY; FLUORESCEIN; FLUORESCEIN GLUCURONIDE; PLASMA FLUORESCENCE; PHARMACOKINETICS; BLOOD RETINAL BARRIER; PERMEABILITY;

D O I：

暂无

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

The evaluation of the blood-ocular barrier for fluorescein requires the measurement of free and unconjugated fluorescein in plasma. This study introduces a new and simple method for the determination of free fluorescein in plasma on the basis of determined total free plasma fluorescense and the free fraction of fluorescence. An excellent good correlation between differential spectrofluorophotometry and this new method is demonstrated. After intravenous administration of sodium fluorescein, the contribution of fluorescein glucuronide to total free plasma fluorescence was evaluated on basis of the areas under the plasma concentration/time curves for fluorescein and fluorescein glucuronide, respectively. After 1 h 8.2% of total free fluorescence in plasma was found to originate from fluorescein glucuronide and after 24 h 18.3% originated from this metabolite. It was concluded that although plasma fluorescein glucuronide measurements are important in the exact evaluation of the blood-ocular barrier, the contribution of fluorescein glucuronide to vitreous fluorescence after intravenous fluorescein administration seems to be of minor magnitude.

引用

页码：447 / 453

页数：7