RADIORECEPTOR ASSAY FOR QUANTIFYING FK-506 IMMUNOSUPPRESSANT IN WHOLE-BLOOD

被引：0

作者：

MURTHY, JN

CHEN, YJ

WARTY, VS

VENKATARAMANAN, R

DONNELLY, JG

ZEEVI, A

SOLDIN, SJ

机构：

[1] CHILDRENS NATL MED CTR,DEPT LAB MED,111 MICHIGAN AVE NW,WASHINGTON,DC 20010

[2] GEORGE WASHINGTON UNIV,SCH MED,DEPT PATHOL,WASHINGTON,DC 20052

[3] GEORGE WASHINGTON UNIV,SCH MED,DEPT PHARMACOL,WASHINGTON,DC 20052

[4] GEORGE WASHINGTON UNIV,SCH MED,DEPT PEDIAT,WASHINGTON,DC 20052

[5] UNIV PITTSBURGH,SCH MED,DEPT PATHOL,PITTSBURGH,PA 15261

[6] UNIV PITTSBURGH,SCH PHARM,PITTSBURGH,PA 15260

来源：

CLINICAL CHEMISTRY | 1992年 / 38卷 / 07期

关键词：

IMMUNOSUPPRESSIVE DRUGS; FK-506 BINDING PROTEIN; ANTIBIOTICS;

D O I：

暂无

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

We describe a quantitative radioreceptor assay (RRA) for quantifying FK-506 in whole blood. FK-506 extracted from whole blood with a cyclohexyl-sorbent column competes with [H-3]dihydro-FK-506 for binding to a partially purified preparation of FK-506 binding protein (FK-BP). Free and protein-bound FK-506 are separated on LH 20 Sephadex chromatographic columns. We compared the results of this method with those of an enzyme immunoassay that uses a monoclonal antibody: r = 0.97, S(y\x) = 0.039. Between-day precisions (CV) at FK-506 concentrations of 8 and 17-mu-g/L were 9.2% and 8.2%, respectively. Within-run precisions were 5.9%, 8.1%, and 9.4%, respectively, at 4, 8, and 15-mu-g/L. Analytical recovery, evaluated at 5, 10, 15, 20, and 25-mu-g/L for FK-506 added to whole blood, ranged from 98% to 103%. The assay can reliably quantify FK-506 blood concentrations between 1.0 and 25-mu-g/L.

引用

页码：1307 / 1310

页数：4