ISOLATION AND CHARACTERIZATION OF A PLANT CDNA SHOWING HOMOLOGY TO ANIMAL GLUTATHIONE PEROXIDASES

被引：155

作者：

CRIQUI, MC ^{[1
]}

JAMET, E ^{[1
]}

PARMENTIER, Y ^{[1
]}

MARBACH, J ^{[1
]}

DURR, A ^{[1
]}

FLECK, J ^{[1
]}

机构：

[1] CNRS,INST BIOL MOLEC PLANTES,CNRS,12 RUE GEN ZIMMER,F-67000 STRASBOURG,FRANCE

来源：

PLANT MOLECULAR BIOLOGY | 1992年 / 18卷 / 03期

关键词：

BTUE; GLUTATHIONE PEROXIDASE; NICOTIANA-SYLVESTRIS; PROTOPLAST; STRESS;

D O I：

10.1007/BF00040684

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA library from freshly isolated protoplasts was differentially screened using cDNAs from mesophyll cells, stressed leaf strips and cell suspension cultures. One of the selected clones, 6P229, turned out to encode a putative polypeptide showing homology to the btuE periplasmic protein of Escherichia coli and to animal selenium-dependent glutathione peroxidases. A major difference was that the putative selenocysteine in the active site was not encoded by the termination codon TGA. The 6P229 gene was found to be expressed in germinating seeds, in apex and in flowers, as well as in stressed tissues. This pattern of expression would be consistent with a key role in cellular metabolism such as defense against oxidative stresses.

引用

页码：623 / 627

页数：5

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