THE ADAPTATION OF 3 ISOLATES OF BABESIA-DIVERGENS TO CONTINUOUS CULTURE IN RAT ERYTHROCYTES

被引：17

作者：

MUSA, NB ^{[1
]}

PHILLIPS, RS ^{[1
]}

机构：

[1] UNIV GLASGOW, WELLCOME LABS EXPTL PARASITOL, BEARSDEN RD, GLASGOW G61 1QH, SCOTLAND

来源：

PARASITOLOGY | 1991年 / 103卷

基金：

英国惠康基金;

关键词：

BABESIA-DIVERGENS; INVITRO CULTURE; ADAPTATION TO RATS;

D O I：

10.1017/S0031182000059436

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Three isolates of Babesia divergens have been cultured continuously for 6 months in rat erythrocytes using the candle jar technique (Trager & Jensen, 1976). One isolate was already rat-adapted, the other two became adapted to rats through continuous culture in rat erythrocytes. Parasites were cultured in rat erythrocytes in RPMI medium supplemented with 20 % foetal calf serum. The highest parasitaemia obtained was 35 % and multiparasitization of red blood cells was often observed. Cultures of B. divergens remained infective to splenectomized rats. Cultures with high parasitaemias contained a large number of extracellular merozoites. When separated from the red blood cells, these extracellular merozoites retained their infectivity.

引用

页码：165 / 170

页数：6

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