SEQUENCE AND EXPRESSION OF RAT ICAM-1

被引：107
作者：
KITA, Y
TAKASHI, T
IIGO, Y
TAMATANI, T
MIYASAKA, M
HORIUCHI, T
机构：
[1] DAIICHI PHARMACEUT CO,TOKYO RES & DEV CTR,MOLEC BIOL RES LAB,KITA KASAI 1 CHOME,EDOGAWA KU,TOKYO 134,JAPAN
[2] DAIICHI PHARMACEUT CO,TOKYO RES & DEV CTR,EXPLORATORY RES LABS 3,TOKYO,JAPAN
[3] TOKYO METROPOLITAN INST MED SCI,DEPT IMMUNOL,TOKYO 113,JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1131卷 / 01期
关键词：
ADHESION MOLECULE; RAT ICAM-1; CDNA;
D O I：
10.1016/0167-4781(92)90107-B
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have isolated cDNA clones-coding for rat intercellular adhesion molecule-1 (RICAM-1) from a cDNA library constructed from rat Ax cells stimulated with IL-1-beta using the mouse ICAM-1 cDNA as a hybridization probe. The RICAM-1 sequence shows 79.1% homology with mouse ICAM-1 and 55.6% homology with human ICAM-1 at the nucleic acid level. In order to examine the expression of RICAM-1 on Chinese hamster ovary (CHO) cells, we constructed the vector, pSV-RICAM1-nco. containing the SV40 promoter. Flowcytometric analysis showed that CHO-K1 cells transfected with pSV-RICAM1-neo expressed high amounts of RICAM-1 on their surfaces.
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页码：108 / 110
页数：3
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