CONSTRUCTION OF PLASMID-FREE DERIVATIVES OF SALMONELLA-TYPHIMURIUM LT2 USING TEMPERATURE-SENSITIVE MUTANTS OF PKZ1 FOR DISPLACEMENT OF THE RESIDENT PLASMID, PSLT

被引：6

作者：

KELLN, RA

LINTOTT, LG

机构：

[1] Department of Chemistry, University of Regina, Regina, S4S 0A2, Saskatchewan

来源：

MOLECULAR & GENERAL GENETICS | 1990年 / 222卷 / 2-3期

关键词：

pKZ1; Plasmid incompatibility; pSLT; Temperature-sensitive mutants;

D O I：

10.1007/BF00633852

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Replication (or partitioning) temperature-sensitive mutants of pKZ1 were isolated and shown to exhibit incompatibility with the resident plasmid (pSLT) of Salmonella typhimurium LT2. Following displacement of pSLT, the mutant plasmids were effectively eliminated from the cell population by passage at 42° C, yielding plasmid-free isolates. © 1990 Springer-Verlag.

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页码：438 / 440

页数：3

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